Editor: Bernard Paul
Phytophthora polonica, a new species isolated from declining Alnus glutinosa stands in Poland
Article first published online: 6 JUL 2006
FEMS Microbiology Letters
Volume 261, Issue 2, pages 165–174, August 2006
How to Cite
Belbahri, L., Moralejo, E., Calmin, G., Oszako, T., García, J. A., Descals, E. and Lefort, F. (2006), Phytophthora polonica, a new species isolated from declining Alnus glutinosa stands in Poland. FEMS Microbiology Letters, 261: 165–174. doi: 10.1111/j.1574-6968.2006.00349.x
- Issue published online: 6 JUL 2006
- Article first published online: 6 JUL 2006
- Received 6 March 2006; revised 28 April 2006; accepted 14 May 2006.First published online 6 july 2006.
- alder decline;
- Phytophthora polonica;
- elongation factor 1α;
- mitochondrial DNA;
- phylogenetic analysis
In a survey of Phytophthora associated with alder decline in Poland, several isolates of a homothallic Phytophthora sp., which could not be assigned to other taxa including Phytophthora alni subspecies, were consistently recovered from rhizosphere soil samples. Their morphology and pathogenicity, as well as sequence data for three nuclear regions (internal transcribed spacer rDNA, elongation factor-1α and β-tubulin) and a coding mitochondrial DNA region (nadh1), were examined. The new Phytophthora species is characterized by the moderate to slow growth rate of its colony in carrot agar at 20°C, high optimal (c. 30°C) and maximum (c. 38°C) growth temperatures, formation of catenulate, often lateral, hyphal swellings, large chlamydospores in agar media and in soil extract, persistent, ovoid to ellipsoid nonpapillate sporangia and large oogonia with paragynous and sometimes amphigynous antheridia. Phytophthora polonica was slightly pathogenic to alder twigs and not pathogenic to trunks of several tree species. In a phylogenetic analysis using either Bayesian inference or maximum likelihood methods, P. polonica falls in clade 8 ‘sensu Kroon et al. (2004)’ of Phytophthora.