Present address: Akira Nozawa, Cell-free Science and Technology Research Center, Ehime University, 3 Bunkyo-Cho, Matsuyama 790-8577, Japan
Roles of BOR1, DUR3, and FPS1 in boron transport and tolerance in Saccharomyces cerevisiae
Article first published online: 4 AUG 2006
FEMS Microbiology Letters
Volume 262, Issue 2, pages 216–222, September 2006
How to Cite
Nozawa, A., Takano, J., Kobayashi, M., Von Wirén, N. and Fujiwara, T. (2006), Roles of BOR1, DUR3, and FPS1 in boron transport and tolerance in Saccharomyces cerevisiae. FEMS Microbiology Letters, 262: 216–222. doi: 10.1111/j.1574-6968.2006.00395.x
Editor: Linda Bisson
- Issue published online: 4 AUG 2006
- Article first published online: 4 AUG 2006
- Received 23 March 2006; revised 23 June 2006; accepted 5 July 2006.First published online 4 August 2006.
- boric acid tolerance;
- boric acid toxicity;
- Saccharomyces cerevisiae;
The roles of three membrane proteins, BOR1, DUR3, and FPS1, in boron (B) transport in yeast were examined. The boron concentration in yeast cells lacking BOR1 was elevated upon exposure to 90 mM boric acid, whereas cells lacking DUR3 or FPS1 showed lower boron concentrations. Compared with control cells, cells overexpressing BOR1 or FPS1 had a lower boron concentration, and cells overexpressing DUR3 had a higher boron concentration. These results suggest that, in addition to the efflux boron transporter BOR1, DUR3 and FPS1 play important roles in regulating the cellular boron concentration. Analysis of the yeast transformants for tolerance to a high boric acid concentration revealed an apparent negative correlation between the protoplasmic boron concentration and the degree of tolerance to a high external boron concentration. Thus, BOR1, DUR3, and FPS1 appear to be involved in tolerance to boric acid and the maintenance of the protoplasmic boron concentration.