Editor: Jose Gil
A genetic and bioinformatic analysis of Streptomyces coelicolor genes containing TTA codons, possible targets for regulation by a developmentally significant tRNA
Article first published online: 14 NOV 2006
FEMS Microbiology Letters
Volume 266, Issue 1, pages 20–28, January 2007
How to Cite
Li, W., Wu, J., Tao, W., Zhao, C., Wang, Y., He, X., Chandra, G., Zhou, X., Deng, Z., Chater, K. F. and Tao, M. (2007), A genetic and bioinformatic analysis of Streptomyces coelicolor genes containing TTA codons, possible targets for regulation by a developmentally significant tRNA. FEMS Microbiology Letters, 266: 20–28. doi: 10.1111/j.1574-6968.2006.00494.x
- Issue published online: 14 NOV 2006
- Article first published online: 14 NOV 2006
- Received 21 May 2006; revised 23 August 2006; accepted 23 August 2006.First published online 14 November 2006.
- TTA codon;
- codon adaptation index
The rarest codon in the high G+C genome of Streptomyces coelicolor is TTA, corresponding in mRNA to the UUA codon that is recognized by a developmentally important tRNA encoded by the bldA gene. There are 145 TTA-containing genes in the chromosome of S. coelicolor. Only 42 of these are represented in the genome of Streptomyces avermitilis, among which only 12 have a TTA codon in both species. The TTA codon is less represented in housekeeping genes and orthologous genes, and is more represented in functional-unknown, extrachromosomal or weakly expressed genes. Twenty one TTA-containing chromosomal genes in S. coelicolor were disrupted, including 12 of the 42 genes that are common to both S. avermitillis and S. coelicolor. None of the mutant strains showed any obvious phenotypic differences from the wild-type strain under tested conditions. Possible reasons for this, and the role and evolution of the observed distribution of TTA codons among Streptomyces genes were discussed.