An acquired efflux system is responsible for copper resistance in Xanthomonas strain IG-8 isolated from China

Authors


  • Present addresses: Robert P. Ryan, BIOMERIT Research Centre, Department of Microbiology, University College Cork, Ireland.

  • Yi-Cheng Sun, Department of Microbiology, University of Alabama at Birmingham, USA.

  • Editor: Skorn Mongkolsuk

Correspondence: David N. Dowling, Department Science & Health, Institute of Technology Carlow, Kilkenny Road, Carlow, Ireland. Tel.: +353 59 9170479; fax: +353 59 9170519; e-mail: dowlingd@itcarlow.ie

Abstract

The genus Xanthomonas contains plant pathogens exhibiting innate resistance to a range of antimicrobial agents. In other genera, multidrug resistance is mediated by a synergy between a low-permeability outer membrane and expression of a number of multidrug efflux systems. This report describes the isolation of a novel gene cluster xmeRSA from Xanthomonas strain IG-8 that mediates copper chloride resistance. Subsequent analysis of these genes showed that they were responsible for the high level of multiple resistance in this strain and were homologues of the sme system of Stenotrophomonas maltophilia. Knock-out mutants of this gene cluster indicate that these genes are required for the copper resistance phenotype of strain IG-8. Expression analysis using lacZ fusions indicates that the genes are regulated by copper and other antimicrobials. Bioinformatic analysis suggests that these genes were acquired by horizontal gene transfer.

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