Editor: Jeff Cole
Species-specific PCR detection of the fish pathogen, Vibrio anguillarum, using the amiB gene, which encodes N-acetylmuramoyl-l-alanine amidase
Article first published online: 26 FEB 2007
FEMS Microbiology Letters
Volume 269, Issue 2, pages 201–206, April 2007
How to Cite
Hong, G.-E., Kim, D.-G., Bae, J.-Y., Ahn, S.-H., Bai, S. C. and Kong, I.-S. (2007), Species-specific PCR detection of the fish pathogen, Vibrio anguillarum, using the amiB gene, which encodes N-acetylmuramoyl-l-alanine amidase. FEMS Microbiology Letters, 269: 201–206. doi: 10.1111/j.1574-6968.2006.00618.x
- Issue published online: 13 MAR 2007
- Article first published online: 26 FEB 2007
- Received 9 August 2006; revised 11 December 2006; accepted 11 December 2006.First published online 26 February 2007.
- Vibrio anguillarum;
- amiB gene;
Vibrio anguillarum is the causative agent of the fish disease vibriosis and is the most intensely studied species of Vibrio. In the present study, specific primers and a PCR assay were designed to detect V. anguillarum. The primers were designed to amplify a 429-bp internal region of the V. anguillarum amiB gene, which encodes the peptidoglycan hydrolase N-acetylmuramoyl-l-alanine amidase. PCR specificity was demonstrated by successful amplification of DNA from V. anguillarum and by the absence of a PCR product from 25 other Vibrio strains and various enteric bacteria. The PCR produced a 429-bp amplified fragment from as little as 1 pg of V. anguillarum DNA. The limit of detection for this PCR technique was c. 20 bacterial colonies in 25 mg of infected flounder tissue. These results suggest that this PCR system is a sensitive and species-specific detection method, and is possible to use as a diagnostic tool to detect V. anguillarum.