Editor: Robert Gunsalus
Identification and analysis of a highly conserved chemotaxis gene cluster in Shewanella species
Article first published online: 21 JUN 2007
FEMS Microbiology Letters
Volume 273, Issue 2, pages 180–186, August 2007
How to Cite
Li, J., Romine, M. F. and Ward, M. J. (2007), Identification and analysis of a highly conserved chemotaxis gene cluster in Shewanella species. FEMS Microbiology Letters, 273: 180–186. doi: 10.1111/j.1574-6968.2007.00810.x
- Issue published online: 21 JUN 2007
- Article first published online: 21 JUN 2007
- Received 9 March 2007; revised 10 April 2007; accepted 1 May 2007.First published online 21 June 2007.
- Shewanella oneidensis;
- Vibrio cholerae;
- Pseudomonas aeruginosa;
A conserved cluster of chemotaxis genes was identified from the genome sequences of fifteen Shewanella species. An in-frame deletion of the cheA-3 gene, which is located in this cluster, was created in S. oneidensis MR-1 and the gene shown to be essential for chemotactic responses to anaerobic electron acceptors. The CheA-3 protein showed strong similarity to Vibrio cholerae CheA-2 and P. aeruginosa CheA-1, two proteins that are also essential for chemotaxis. The genes encoding these proteins were shown to be located in chemotaxis gene clusters closely related to the cheA-3-containing cluster in Shewanella species. The results of this study suggest that a combination of gene neighborhood and homology analyses may be used to predict which cheA genes are essential for chemotaxis in groups of closely related microorganisms.