Present address: Aaron M. Saunders, Danish Technological Institute, Kongsvang Allé 29, Aarhus C, 8000, Denmark.
Proton motive force generation from stored polymers for the uptake of acetate under anaerobic conditions
Article first published online: 4 JUL 2007
FEMS Microbiology Letters
Volume 274, Issue 2, pages 245–251, September 2007
How to Cite
Saunders, A. M., Mabbett, A. N., McEwan, A. G. and Blackall, L. L. (2007), Proton motive force generation from stored polymers for the uptake of acetate under anaerobic conditions. FEMS Microbiology Letters, 274: 245–251. doi: 10.1111/j.1574-6968.2007.00839.x
Editor: Anthony George
- Issue published online: 4 JUL 2007
- Article first published online: 4 JUL 2007
- Received 23 April 2007; revised 7 June 2007; accepted 8 June 2007.First published online 5 July 2007.
- enhanced biological phosphorus removal;
- secondary transport;
- substrate uptake
The bacteria facilitating enhanced biological phosphorus removal gain a selective advantage from intracellularly stored polymer-driven substrate uptake under anaerobic conditions during sequential anaerobic : aerobic cycling. Mechanisms for these unusual membrane transport processes were proposed and experimentally validated using selective inhibitors and highly-enriched cultures of a polyphosphate-accumulating organism, Accumulibacter, and a glycogen-accumulating organism, Competibacter. Acetate uptake by both Accumulibacter and Competibacter was driven by a proton motive force (PMF). Stored polymers were used to generate the PMF –Accumulibacter used phosphate efflux through the Pit transporter, while Competibacter generated a PMF by proton efflux through the ATPase and fumarate reductase in the reductive TCA cycle.