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Complete nucleotide sequence of a virulence plasmid of Salmonella enterica serovar Dublin and its phylogenetic relationship to the virulence plasmids of serovars Choleraesuis, Enteritidis and Typhimurium

  1. Seau-Feng Hong1,
  2. Cheng-Hsun Chiu2,
  3. Chishih Chu3,
  4. Ye Feng4,
  5. Jonathan T. Ou1,†

Article first published online: 10 MAR 2008

DOI: 10.1111/j.1574-6968.2008.01096.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 282, Issue 1, pages 39–43, May 2008

Additional Information

How to Cite

Hong, S.-F., Chiu, C.-H., Chu, C., Feng, Y. and Ou, J. T. (2008), Complete nucleotide sequence of a virulence plasmid of Salmonella enterica serovar Dublin and its phylogenetic relationship to the virulence plasmids of serovars Choleraesuis, Enteritidis and Typhimurium. FEMS Microbiology Letters, 282: 39–43. doi: 10.1111/j.1574-6968.2008.01096.x

Author Information

  1. 1

    Department of Microbiology and Immunology, Chang Gung University College of Medicine, Taoyuan, Taiwan

  2. 2

    Department of Pediatrics, Chang Gung Children's Hospital, Chang Gung University College of Medicine, Taoyuan, Taiwan

  3. 3

    Department of Microbiology and Immunology, National Chiayi University, Chiayi, Taiwan

  4. 4

    James D. Watson Institute of Genome Sciences, Zhejiang University, Hangzhou, PR China

  1. †

    ✠Deceased.

*Correspondence: Cheng-Hsun Chiu, Department of Pediatrics, Chang Gung Children's Hospital, Chang Gung University College of Medicine, 5 Fu-Hsin Street, Kweishan 333, Taoyuan, Taiwan. Tel.: +886 3 3281200; fax: +886 3 3288957; e-mail: chchiu@adm.cgmh.org.tw

Publication History

  1. Issue published online: 18 MAR 2008
  2. Article first published online: 10 MAR 2008
  3. Received 24 October 2007; accepted 16 January 2008.First published online 10 March 2008.

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Keywords:

  • complete nucleotide sequence;
  • Salmonella enterica;
  • Salmonella enterica serovar Dublin;
  • virulence plasmid

Abstract

The complete nucleotide sequence of pOU1113 (pSDVu), one of the two types of virulence plasmids of Salmonella enterica serovar Dublin, was determined. It contained 80 156 bp with 53.8 mol% G+C content. Approximately 70 genes could be discerned. Compared with pSTV, the virulence plasmid of serovar Typhimurium, pOU1113 was shorter owing to a missing region amounting to c. 10 kb; furthermore, except for a unique 10 849-bp region, the nucleotide as well as deduced amino acid sequences of pOU1113 were nearly identical to the corresponding regions of three S. enterica virulence plasmids, namely pSCV (virulence plasmid of Choleraesuis), pSTV and pSEV (virulence plasmids of Enteritidis), confirming their close phylogenetic relationship. Comparative analysis indicated that these virulence plasmids appeared to have descended by deletion from a relatively large plasmid to smaller ones, with some recombination events occurring over time. From a biological and evolutionary point of view, if the decreasing sizes of pOU1113 and pSCV truly reflect a process in which the virulence plasmid has been shedding unnecessary genes during evolution, our data suggest that some genes in the missing region, such as the pef and tra operons, could have a minimal role in maintaining the survival of the bacteria in their environmental niche.

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