Present addresses: Sharon A. Huws, Department of Plant, Animal and Microbial Science, Institute of Grassland and Environmental Research (IGER), Plas Gogerddan, Aberystwyth, SY23 3EB, UK. Michael R.W. Brown, Department of Pharmacy, School of Applied Sciences, University of Wolverhampton, Wulfruna street, Wolverhampton, WV1 1SB, UK.
Interactions of some common pathogenic bacteria with Acanthamoeba polyphaga
Article first published online: 9 APR 2008
© 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 282, Issue 2, pages 258–265, May 2008
How to Cite
Huws, S. A., Morley, R. J., Jones, M. V., Brown, M. R. W. and Smith, A. W. (2008), Interactions of some common pathogenic bacteria with Acanthamoeba polyphaga. FEMS Microbiology Letters, 282: 258–265. doi: 10.1111/j.1574-6968.2008.01123.x
Editor: Pauline Schaap
- Issue published online: 9 APR 2008
- Article first published online: 9 APR 2008
- Received 13 September 2007; revised 20 December 2007; accepted 12 February 2008.First published online April 2008.
- Staphylococcus aureus;
- Acanthamoeba polyphaga;
- protozoa–bacteria interactions
Protozoan grazing is a major trophic pathway whereby the biomass re-enters the food web. Nonetheless, not all bacteria are digested by protozoa and the number known to evade digestion, resulting in their environmental augmentation, is increasing. We investigated the interactions of Bacillus cereus, Enterococcus faecalis, Enteropathogenic Escherichia coli (EPEC), Listeria monocytogenes, Salmonella enterica serovar Typhimurium, and methicillin-sensitive Staphylococcus aureus (MSSA), with the amoeba, Acanthamoeba polyphaga. There was evidence of predation of all bacterial species except L. monocytogenes and S. aureus, where extracellular numbers were significantly higher when cultured with amoebae compared with growth in the absence of amoebae. Intracellular growth kinetic experiments and fluorescent confocal microscopy suggest that S. aureus survived and may even multiply within A. polyphaga, whereas there was no apparent intra-amoebal replication of L. monocytogenes and higher numbers were likely sustained on metabolic waste products released during coculture.