The Pmr1 protein, the major yeast Ca2+-ATPase in the Golgi, regulates intracellular levels of the cadmium ion

Authors

  • Cláudio Marcos Lauer Júnior,

    1. Departamento de Biofísica/Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
    2. Universitário Feevale, Campus II, Novo Hamburgo, RS, Brazil
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  • Diego Bonatto,

    1. Instituto de Biotecnologia, Universidade de Caxias do Sul (UCS), Bloco, Caxias do Sul, RS, Brazil
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  • Albanin Aparecida Mielniczki-Pereira,

    1. Departamento de Biofísica/Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
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  • Ana Zilles Schuch,

    1. Departamento de Biofísica/Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
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  • Johnny Ferraz Dias,

    1. Instituto de Física, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
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  • Maria-Lúcia Yoneama,

    1. Instituto de Física, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
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  • João Antonio Pêgas Henriques

    1. Departamento de Biofísica/Centro de Biotecnologia, Universidade Federal do Rio Grande do Sul (UFRGS), Porto Alegre, RS, Brazil
    2. Instituto de Biotecnologia, Universidade de Caxias do Sul (UCS), Bloco, Caxias do Sul, RS, Brazil
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  • Editor: Derek Jamieson

Correspondence: João Antonio Pêgas Henriques, Departamento de Biofísica/Centro de Biotecnologia, UFRGS, Av. Bento Gonçalves 9500, Porto Alegre, RS, Brazil, 91507-970. Tel.: +55 51 3308 7602; fax: +55 51 3308 6084; e-mail: pegas@cbiot.ufrgs.br

Abstract

Cadmium is a nonessential, highly toxic heavy metal that shows ionic properties similar to calcium. These ionic similarities imply that the cadmium ion, Cd2+, is a calcium ion, Ca2+, receptor-agonist, affecting the same biochemical pathways involved in Ca2+ homeostasis. In the yeast Saccharomyces cerevisiae, the PMC1 and PMR1 genes encode vacuolar and Golgi Ca2+-ATPases, respectively. The PMR1 protein product Pmr1p is involved in both Ca2+ and Mn2+ homeostasis. This study investigated the importance of Pmc1p and Pmr1p for Cd2+ cellular detoxification. Using the standard techniques of yeast molecular research and a multielemental procedure named particle-induced X-ray emission, Pmr1p was identified as a protein that directly participates in the detoxification of Cd2+, possibly through the secretory pathway. The results allow us to posit a model of Cd2+ detoxification where Pmr1p has a central role in cell survival in a Cd2+-rich environment.

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