Editor: Mark Enright
High-intensity narrow-spectrum light inactivation and wavelength sensitivity of Staphylococcus aureus
Article first published online: 28 JUN 2008
© 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 285, Issue 2, pages 227–232, August 2008
How to Cite
Maclean, M., MacGregor, S. J., Anderson, J. G. and Woolsey, G. (2008), High-intensity narrow-spectrum light inactivation and wavelength sensitivity of Staphylococcus aureus. FEMS Microbiology Letters, 285: 227–232. doi: 10.1111/j.1574-6968.2008.01233.x
- Issue published online: 14 JUL 2008
- Article first published online: 28 JUN 2008
- Received 19 December 2007; accepted 15 May 2008.First published online 16 June 2008.
- photodynamic inactivation;
- visible light;
- Staphylococcus aureus;
- wavelength sensitivity
This study was conducted to investigate the bactericidal effects of visible light on methicillin-sensitive and methicillin-resistant Staphylococcus aureus (MRSA), and subsequently identify the wavelength sensitivity of S. aureus, in order to establish the wavelengths inducing maximum inactivation. Staphylococcus aureus, including MRSA strains, were shown to be inactivated by exposure to high-intensity visible light, and, more specifically, through a series of studies using a xenon broadband white-light source in conjunction with a selection of optical filters, it was found that inactivation of S. aureus occurs upon exposure to blue light of wavelengths between 400 and 420 nm, with maximum inactivation occurring at 405±5 nm. This visible-light inactivation was achieved without the addition of exogenous photosensitisers. The significant safety benefit of these blue-light wavelengths over UV light, in addition to their ability to inactivate medically important microorganisms such as MRSA, emphasises the potential of exploiting these non-UV wavelengths for disinfection applications.