Editor: Anthony George
Localization of the ActIII actinorhodin polyketide ketoreductase to the cell wall
Article first published online: 7 AUG 2008
© 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 287, Issue 1, pages 15–21, October 2008
How to Cite
Xu, X.-P., Wang, Z.-J., Fan, K.-Q., Wang, S.-L., Jia, C.-J., Han, H., Ramalingam, E. and Yang, K.-Q. (2008), Localization of the ActIII actinorhodin polyketide ketoreductase to the cell wall. FEMS Microbiology Letters, 287: 15–21. doi: 10.1111/j.1574-6968.2008.01289.x
- Issue published online: 1 SEP 2008
- Article first published online: 7 AUG 2008
- Received 26 April 2008; accepted 15 June 2008.First published online 7 August 2008.
- Streptomyces coelicolor;
- cell wall
Structurally diverse polyketides provide a rich reservoir of bioactive molecules. Actinorhodin, a model aromatic polyketide, is synthesized by minimal type II polyketide synthase and tailoring enzymes. The ActIII actinorhodin ketoreductase is a key tailoring enzyme in actinorhodin biosynthesis. With purified antibodies against actinorhodin polyketide synthase α subunit (KSα) and ketoreductase, we conducted systematic localization experiments of the two proteins in Streptomyces coelicolor subproteomes. The results support the membrane location of KSα and cell-wall location of ketoreductase. Considering previous evidence that some other tailoring enzymes of actinorhodin biosynthesis may be located outside the cytoplasm, a picture is emerging of an extensive role for extracellular biochemistry in the synthesis of type II polyketide antibiotic.