Editor: Masao Mitsuyama
Differences in nitrate reduction between Mycobacterium tuberculosis and Mycobacterium bovis are due to differential expression of both narGHJI and narK2
Article first published online: 8 DEC 2008
Journal compilation © 2008 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. No claim to original US government works
FEMS Microbiology Letters
Volume 290, Issue 2, pages 129–134, January 2009
How to Cite
Sohaskey, C. D. and Modesti, L. (2009), Differences in nitrate reduction between Mycobacterium tuberculosis and Mycobacterium bovis are due to differential expression of both narGHJI and narK2. FEMS Microbiology Letters, 290: 129–134. doi: 10.1111/j.1574-6968.2008.01424.x
- Issue published online: 8 DEC 2008
- Article first published online: 8 DEC 2008
- Received 10 June 2008; accepted 5 October 2008.First published online December 2008.
- nitrate reductase;
Both Mycobacterium tuberculosis and Mycobacterium bovis can produce tuberculosis in humans. Mycobacterium tuberculosis has low nitrate reductase activity during aerobic growth (AG), but shows strong hypoxic induction. Virulent M. bovis has weak activity during AG with no hypoxic induction. Bacille Calmette–Guerin (BCG) lacks activity in both stages. Transcription of narG of the nitrate reductase enzyme operon was higher in M. tuberculosis than in M. bovis or BCG. Transcription of narK2 encoding the nitrate transporter was induced by hypoxia in M. tuberculosis but not M. bovis or BCG. Insertion of the M. tuberculosis narGHJI operon into M. bovis resulted in increased activity only during AG. Regulation of both the nitrate reductase enzyme and transporter are regulated differently in the two species.