Editor: Marco Soria
The GO system prevents ROS-induced mutagenesis and killing in Pseudomonas aeruginosa
Article first published online: 9 MAR 2009
© 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 294, Issue 1, pages 89–96, May 2009
How to Cite
Sanders, L. H., Sudhakaran, J. and Sutton, M. D. (2009), The GO system prevents ROS-induced mutagenesis and killing in Pseudomonas aeruginosa. FEMS Microbiology Letters, 294: 89–96. doi: 10.1111/j.1574-6968.2009.01550.x
- Issue published online: 2 APR 2009
- Article first published online: 9 MAR 2009
- Received 6 February 2009; accepted 11 February 2009.First published online 9 March 2009.
- base excision repair;
- DNA damage;
- reactive oxygen species;
Inactivation of the Pseudomonas aeruginosa mutM, mutY, or mutT gene conferred a 2.4-, 17.2-, or 38.1-fold increase in spontaneous mutation frequency, respectively. Importantly, the mutY and mutT strains each displayed a robust H2O2-induced mutation frequency. In addition, the mutM, mutY, and mutT mutations severely sensitized P. aeruginosa to killing by H2O2, suggesting that these gene products act to repair one or more cytotoxic lesions in P. aeruginosa. Nucleotide sequence analysis of a fragment of the rpoB gene from rifampicin resistant mutM-, mutY-, and, mutT-deficient strains was consistent with this conclusion. These findings are discussed in terms of possible roles for mutM, mutY, and mutT in contributing to survival and mutagenesis of P. aeruginosa colonizing the airways of cystic fibrosis patients.