Present address: Yoon-Suk Kang, Division of Environmental Science and Ecological Engineering, Korea University, Anam-Dong 5 Ga, Seoul 136-713, Korea.
Antibacterial activity of a disaccharide isolated from Streptomyces sp. strain JJ45 against Xanthomonas sp.
Article first published online: 18 MAR 2009
© 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 294, Issue 1, pages 119–125, May 2009
How to Cite
Kang, Y.-S., Lee, Y., Cho, S. K., Lee, K.-H., Kim, B.-J., Kim, M., Lim, Y. and Cho, M. (2009), Antibacterial activity of a disaccharide isolated from Streptomyces sp. strain JJ45 against Xanthomonas sp. FEMS Microbiology Letters, 294: 119–125. doi: 10.1111/j.1574-6968.2009.01561.x
Editor: Gilbert Shama
- Issue published online: 2 APR 2009
- Article first published online: 18 MAR 2009
- Received 28 October 2008; accepted 24 February 2009.First published online 18 March 2009.
- antibacterial activity;
- α-l-sorbofuranose (32)-β-d-altrofuranose
Of the 316 actinomycetes strains isolated from various habitats, Streptomyces sp. strain JJ45 showed the strongest antibiotic activity against the plant pathogenic bacteria Xanthomonas campestris pv. campestris and was thus chosen for further study. The 16S rRNA gene sequence (1500 bp) and rpoB gene partial sequence (306 bp) of Streptomyces strains JJ45A and JJ45B were determined. The respective strain JJ45B sequences exhibited 96.8% identity with the Streptococcus gelaticus 16S rRNA gene sequence and 98.4% identity with the Streptococcus vinaceus ATCC 27478 rpoB partial sequence. The fermentation broth of the JJ45B strain was extracted to find an inhibitor of bacterial growth. The distilled water extract showed the highest activity against pathogenic bacteria. The active molecule was isolated by column chromatography on polyacrylamide or silica gel, thin-layer chromatography, and HPLC. It showed growth inhibition activity only toward phytopathogenic Xanthomonas sp. The structure of the compound was identified as α-l-sorbofuranose (32)-β-d-altrofuranose based on the interpretation of the nuclear magnetic resonance spectra.