Editor: Rustam Aminov
Novel persistence genes in Pseudomonas aeruginosa identified by high-throughput screening
Article first published online: 21 MAY 2009
© 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 297, Issue 1, pages 73–79, August 2009
How to Cite
De Groote, V. N., Verstraeten, N., Fauvart, M., Kint, C. I., Verbeeck, A. M., Beullens, S., Cornelis, P. and Michiels, J. (2009), Novel persistence genes in Pseudomonas aeruginosa identified by high-throughput screening. FEMS Microbiology Letters, 297: 73–79. doi: 10.1111/j.1574-6968.2009.01657.x
- Issue published online: 2 JUL 2009
- Article first published online: 21 MAY 2009
- Received 24 March 2009; accepted 8 May 2009.Final version published online 8 June 2009.
- antibiotic tolerance;
- stationary phase;
Persister cells are phenotypic variants that are extremely tolerant to high concentrations of antibiotics. They constitute a fraction of stationary phase cultures and biofilm populations of numerous bacterial species, such as the opportunistic pathogen Pseudomonas aeruginosa. Even though persisters are believed to be an important cause of incomplete elimination of infectious populations by antibiotics, their nature remains obscure. Most studies on persistence have focused on the model organism Escherichia coli and only a limited number of persistence genes have been identified to date. We performed the first large-scale screening of a P. aeruginosa PA14 mutant library to identify novel genes involved in persistence. A total of 5000 mutants were screened in a high-throughput manner and nine new persistence mutants were identified. Four mutants (with insertions in dinG, spuC, PA14_17880 and PA14_66140) exhibited a low persister phenotype and five mutants (in algR, pilH, ycgM, pheA and PA14_13680) displayed high persistence. These genes may serve as new candidate drug targets in the combat against P. aeruginosa infections.