Editor: Roger Buxton
The Escherichia coli CsrB and CsrC small RNAs are strongly induced during growth in nutrient-poor medium
Article first published online: 21 MAY 2009
© 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 297, Issue 1, pages 80–86, August 2009
How to Cite
Jonas, K. and Melefors, Ö. (2009), The Escherichia coli CsrB and CsrC small RNAs are strongly induced during growth in nutrient-poor medium. FEMS Microbiology Letters, 297: 80–86. doi: 10.1111/j.1574-6968.2009.01661.x
- Issue published online: 2 JUL 2009
- Article first published online: 21 MAY 2009
- Received 4 March 2009; accepted 14 May 2009.Final version published online 16 June 2009.
- small RNAs;
- BarA-UvrY two-component system;
- external growth conditions
The carbon storage regulatory (Csr) system is a complex network controlling various phenotypes in many eubacteria. So far, the external conditions by which the system is regulated are poorly understood. Here we show that the expression of the two noncoding small RNAs CsrB and CsrC in Escherichia coli is strongly increased in cultures grown in minimal medium. Addition of tryptone, casamino acids or a mixture of amino acids to a culture grown in minimal medium led to a rapid reduction in the levels of CsrB. Based on this we propose that the expression of the Csr sRNAs is controlled by the amino acid availability in the growth medium.