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Genome-wide identification of novel genomic islands that contribute to Salmonella virulence in mouse systemic infection
Article first published online: 10 JUN 2009
DOI: 10.1111/j.1574-6968.2009.01686.x
© 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
Additional Information
How to Cite
Haneda, T., Ishii, Y., Danbara, H. and Okada, N. (2009), Genome-wide identification of novel genomic islands that contribute to Salmonella virulence in mouse systemic infection. FEMS Microbiology Letters, 297: 241–249. doi: 10.1111/j.1574-6968.2009.01686.x
Publication History
- Issue published online: 10 JUL 2009
- Article first published online: 10 JUN 2009
- Received 4 December 2008; accepted 5 June 2009.Final version published online 3 July 2009.
Keywords:
- Salmonella infection;
- virulence;
- genomic island;
- tRNA;
- horizontal gene transfer
Abstract
Salmonella pathogenicity islands are inserted into the genome by horizontal gene transfer and are required for expression of full virulence. Here, we performed tRNA scanning of the genome of Salmonella enterica serovar Typhimurium and compared it with that of nonpathogenic Escherichia coli in order to identify genomic islands that contribute to Salmonella virulence. Using deletion analysis, we identified four genomic islands that are required for virulence in the mouse infection model. One of the newly identified pathogenicity islands was the pheV-tRNA-located genomic island, which is comprised of 26 126 bp, and encodes 22 putative genes, including STM3117–STM3138. We also showed that the pheV tRNA-located genomic island is widely distributed among different nontyphoid Salmonella serovars. Furthermore, genes including STM3118–STM3121 were identified as novel virulence-associated genes within the pheV-tRNA-located genomic island. These results indicate that a Salmonella-specific pheV-tRNA genomic island is involved in Salmonella pathogenesis among the nontyphoid Salmonella serovars.