Editor: Reggie Lo
Mini-Tn7 vectors as genetic tools for gene cloning at a single copy number in an industrially important and phytopathogenic bacteria, Xanthomonas spp.
Article first published online: 26 JUN 2009
© 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 298, Issue 1, pages 111–117, September 2009
How to Cite
Jittawuttipoka, T., Buranajitpakorn, S., Fuangthong, M., Schweizer, H. P., Vattanaviboon, P. and Mongkolsuk, S. (2009), Mini-Tn7 vectors as genetic tools for gene cloning at a single copy number in an industrially important and phytopathogenic bacteria, Xanthomonas spp. FEMS Microbiology Letters, 298: 111–117. doi: 10.1111/j.1574-6968.2009.01707.x
- Issue published online: 20 JUL 2009
- Article first published online: 26 JUN 2009
- Received 4 March 2009; accepted 17 June 2009.Final version published online 16 July 2009.
- single copy number gene cloning;
- unmarked mutation;
Transposon mini-Tn7 vectors insert into the chromosome of several Gram-negative bacteria in a site-specific manner. Here, we showed the application of mini-Tn7 as single copy site-specific integration vector system for Xanthomonas campestris pv. campestris. The transposition of the mini-Tn7 into the bacterial genome was detected at a Tn7 attachment (attTn7) site located downstream of glmS1. Furthermore, using a newly constructed vector pBBR1FLP2 containing the flipase (FLP) recombinase for site-specific excision of the sequence between the FLP recombinase target (FRT) sites, and a sacB counter selection marker, an unmarked mini-Tn7 insertion mutant was created. Mini-Tn7 insertion did not affect bacterial virulence on the tested plant. The mini-Tn7 and FLP–FRT systems also work well in Xanthomonas oryzae pv. oryzae.