Editor: Ian Henderson
Next generation sequencing of microbial transcriptomes: challenges and opportunities
Article first published online: 21 AUG 2009
© 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 302, Issue 1, pages 1–7, January 2010
How to Cite
Van Vliet, A. H.M. (2010), Next generation sequencing of microbial transcriptomes: challenges and opportunities. FEMS Microbiology Letters, 302: 1–7. doi: 10.1111/j.1574-6968.2009.01767.x
- Issue published online: 27 NOV 2009
- Article first published online: 21 AUG 2009
- Received 10 June 2009; accepted 15 August 2009.Final version published online 4 September 2009.
- microbial transcriptomes;
- high-throughput sequencing;
- cDNA libraries;
Over the past 15 years, microbial functional genomics has been made possible by the combined power of genome sequencing and microarray technology. However, we are now approaching the technical limits of microarray technology, and microarrays are now being superseded by transcriptomics based on high-throughput (next generation) DNA-sequencing technologies. The term RNA-seq has been coined to represent transcriptomics by next-generation sequencing. Although pioneered on eukaryotic organisms due to the relative ease of working with eukaryotic mRNA, the RNA-seq technology is now being ported to microbial systems. This review will discuss the opportunities of RNA-seq transcriptome sequencing for microorganisms, and also aims to identify challenges and pitfalls of the use of this new technology in microorganisms.