Editor: Craig Winstanley
Identification of Salmonella enterica subspecies I, Salmonella enterica serovars Typhimurium, Enteritidis and Typhi using multiplex PCR
Article first published online: 3 OCT 2009
© 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 301, Issue 1, pages 137–146, December 2009
How to Cite
Park, S. H., Kim, H. J., Cho, W. H., Kim, J. H., Oh, M. H., Kim, S. H., Lee, B. K., Ricke, S. C. and Kim, H. Y. (2009), Identification of Salmonella enterica subspecies I, Salmonella enterica serovars Typhimurium, Enteritidis and Typhi using multiplex PCR. FEMS Microbiology Letters, 301: 137–146. doi: 10.1111/j.1574-6968.2009.01809.x
Present address: Si Hong Park, Department of Food Science, Center for Food Safety, University of Arkansas, Fayetteville, AR 72704-5690, USA.
- Issue published online: 2 NOV 2009
- Article first published online: 3 OCT 2009
- Received 13 May 2009; accepted 24 September 2009.Final version published online 16 October 2009.
- Salmonella enterica serovar Typhi;
- Salmonella enterica serovar Typhimurium;
- mutiplex PCR;
- comparative genomics
This study was designed to develop a multiplex PCR method with five specific primer pairs for the detection of Salmonella spp., Salmonella subspecies I, Salmonella enterica serovars Typhimurium, Typhi and Enteritidis. A multiplex PCR was constructed with five primer pairs for the detection of Salmonella and pathogenic Salmonella serovars, including a specific primer pair for Salmonella Typhi, based on the sequence comparison between genomic DNA sequences of 12 Salmonella strains. Each primer pair was specifically targeted to Salmonella spp., Salmonella subspecies I, Salmonella Typhimurium, Typhi and Enteritidis. This multiplex PCR was evaluated with various DNAs of Salmonella serovars that yielded high specificity for amplifying the expected PCR products of Salmonella serovars. Using this primer pair, a set of multiplex PCR was performed for the rapid identification of salmonellae and major pathogenic Salmonella serovars. Although this multiplex PCR method will need to be evaluated for a wide range of Salmonella serovars among multilaboratories, it should be useful for identifying clinically significant strains of Salmonella serovars rapidly and accurately without the need for serological testing.