You have full text access to this OnlineOpen article

Skn1 and Ipt1 negatively regulate autophagy in Saccharomyces cerevisiae

  1. Karin Thevissen1,
  2. Wei-Lien Yen2,
  3. Didac Carmona-Gutierrez3,
  4. Jolanta Idkowiak-Baldys4,
  5. An M. Aerts1,
  6. Isabelle E.J.A. François1,
  7. Frank Madeo3,
  8. Daniel J. Klionsky2,
  9. Yusuf A. Hannun4,
  10. Bruno P.A. Cammue1

Article first published online: 27 NOV 2009

DOI: 10.1111/j.1574-6968.2009.01869.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 303, Issue 2, pages 163–168, February 2010

Additional Information

How to Cite

Thevissen, K., Yen, W.-L., Carmona-Gutierrez, D., Idkowiak-Baldys, J., Aerts, A. M., François, I. E.J.A., Madeo, F., Klionsky, D. J., Hannun, Y. A. and Cammue, B. P.A. (2010), Skn1 and Ipt1 negatively regulate autophagy in Saccharomyces cerevisiae. FEMS Microbiology Letters, 303: 163–168. doi: 10.1111/j.1574-6968.2009.01869.x

Author Information

  1. 1

    Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Heverlee, Belgium

  2. 2

    Life Sciences Institute, University of Michigan, Ann Arbor, MI, USA

  3. 3

    Institute of Molecular Biosciences (IMB), Karl-Franzens University, Graz, Austria

  4. 4

    Department of Biochemistry and Molecular Biology, Medical University of South Carolina, Charleston, SC, USA

*Correspondence: Karin Thevissen, Centre of Microbial and Plant Genetics (CMPG), Katholieke Universiteit Leuven, Kasteelpark Arenberg 20, B-3001 Heverlee, Belgium. Tel.: +32 16 32 96 88; fax: +32 16 32 19 66; e-mail: karin.thevissen@biw.kuleuven.be

  1. Editor: Linda Bisson

Publication History

  1. Issue published online: 15 JAN 2010
  2. Article first published online: 27 NOV 2009
  3. Received 13 August 2009; revised 20 November 2009; accepted 23 November 2009.Final version published online 18 December 2009.

SEARCH

SEARCH BY CITATION

ARTICLE TOOLS

View Full Article (HTML) Get PDF (167K)

Keywords:

  • autophagy;
  • DNA fragmentation;
  • apoptosis;
  • sphingolipid

Abstract

We demonstrated that a yeast deletion mutant in IPT1 and SKN1, encoding proteins involved in the biosynthesis of mannosyldiinositolphosphoryl ceramides, is characterized by increased autophagy and DNA fragmentation upon nitrogen (N) starvation as compared with the single deletion mutants or wild type (WT). Apoptotic features were not significantly different between single and double deletion mutants upon N starvation, pointing to increased autophagy in the double Δipt1Δskn1 deletion mutant independent of apoptosis. We observed increased basal levels of phytosphingosine in membranes of the double Δipt1Δskn1 deletion mutant as compared with the single deletion mutants or WT. These data point to a negative regulation of autophagy by both Ipt1 and Skn1 in yeast, with a putative involvement of phytosphingosine in this process.

View Full Article (HTML) Get PDF (167K)