Editor: Linda Bisson
Skn1 and Ipt1 negatively regulate autophagy in Saccharomyces cerevisiae
Article first published online: 27 NOV 2009
© 2009 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 303, Issue 2, pages 163–168, February 2010
How to Cite
Thevissen, K., Yen, W.-L., Carmona-Gutierrez, D., Idkowiak-Baldys, J., Aerts, A. M., François, I. E.J.A., Madeo, F., Klionsky, D. J., Hannun, Y. A. and Cammue, B. P.A. (2010), Skn1 and Ipt1 negatively regulate autophagy in Saccharomyces cerevisiae. FEMS Microbiology Letters, 303: 163–168. doi: 10.1111/j.1574-6968.2009.01869.x
- Issue published online: 15 JAN 2010
- Article first published online: 27 NOV 2009
- Received 13 August 2009; revised 20 November 2009; accepted 23 November 2009.Final version published online 18 December 2009.
- DNA fragmentation;
We demonstrated that a yeast deletion mutant in IPT1 and SKN1, encoding proteins involved in the biosynthesis of mannosyldiinositolphosphoryl ceramides, is characterized by increased autophagy and DNA fragmentation upon nitrogen (N) starvation as compared with the single deletion mutants or wild type (WT). Apoptotic features were not significantly different between single and double deletion mutants upon N starvation, pointing to increased autophagy in the double Δipt1Δskn1 deletion mutant independent of apoptosis. We observed increased basal levels of phytosphingosine in membranes of the double Δipt1Δskn1 deletion mutant as compared with the single deletion mutants or WT. These data point to a negative regulation of autophagy by both Ipt1 and Skn1 in yeast, with a putative involvement of phytosphingosine in this process.