Editor: Craig Shoemaker
Acylhomoserine lactone production and degradation by the fish pathogen Tenacibaculum maritimum, a member of the Cytophaga–Flavobacterium–Bacteroides (CFB) group
Article first published online: 28 DEC 2009
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 304, Issue 2, pages 131–139, March 2010
How to Cite
Romero, M., Avendaño-Herrera, R., Magariños, B., Cámara, M. and Otero, A. (2010), Acylhomoserine lactone production and degradation by the fish pathogen Tenacibaculum maritimum, a member of the Cytophaga–Flavobacterium–Bacteroides (CFB) group. FEMS Microbiology Letters, 304: 131–139. doi: 10.1111/j.1574-6968.2009.01889.x
Present address: Rubén Avendaño-Herrera, Departamento de ciencias biológicas, Facultad de ciencias biológicas, Universidad de Andrés Bello, Santiago, Chile.
- Issue published online: 12 FEB 2010
- Article first published online: 28 DEC 2009
- Received 29 September 2009; revised 9 December 2009; accepted 15 December 2009.Final version published online February 2010.
- quorum sensing;
- quorum quenching;
- acylhomoserine lactone;
Tenacibaculum maritimum (formerly Flexibacter maritimus) is a filamentous, biofilm-forming member of the Cytophaga–Flavobacterium–Bacteroides group (or Bacteroidetes), which causes the widely distributed marine fish disease tenacibaculosis. A search for N-acylhomoserine lactones (AHLs) quorum-sensing (QS) signals in the culture media of nine representative strains of this species using different biosensor strains revealed the presence of short-type AHL activity in all of them. N-butyryl-l-homoserine lactone (C4-HSL) was identified in T. maritimum NCIMB2154T by LC-MS. A degradation activity for long-acyl AHLs (C10-HSL) was subsequently demonstrated in T. maritimum NCIMB2154T. The acidification of the culture medium after degradation did not allow the recovery of C10-HSL, which indicates a possible acylase-type degradation activity. Even though the physiological processes under the control of AHL-mediated QS in T. maritimum need to be further characterized, this discovery extends the paradigm of AHL-mediated QS signalling beyond the Proteobacteria and reinforces its ecological significance.