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Keywords:

  • Mycobacterium ulcerans;
  • Buruli ulcer;
  • real-time PCR;
  • Ashanti region;
  • environmental samples;
  • small mammals

Abstract

This study reports the first successful application of real-time PCR for the detection of Mycobacterium ulcerans, the causative agent of Buruli ulcer (BU), in Ghana, a BU-endemic country. Environmental samples and organs of small mammals were analyzed. The real-time PCR assays confirmed the presence of M. ulcerans in a water sample collected in a BU-endemic village in the Ashanti Region.