Editor: Jan-Ingmar Flock
Biochemical characteristics among Mycobacterium bovis BCG substrains
Article first published online: 6 APR 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 306, Issue 2, pages 103–109, May 2010
How to Cite
Hayashi, D., Takii, T., Mukai, T., Makino, M., Yasuda, E., Horita, Y., Yamamoto, R., Fujiwara, A., Kanai, K., Kondo, M., Kawarazaki, A., Yano, I., Yamamoto, S. and Onozaki, K. (2010), Biochemical characteristics among Mycobacterium bovis BCG substrains. FEMS Microbiology Letters, 306: 103–109. doi: 10.1111/j.1574-6968.2010.01947.x
- Issue published online: 16 APR 2010
- Article first published online: 6 APR 2010
- Received 11 November 2009; revised 8 February 2010; accepted 18 February 2010.Final version published online April 2010.
- biochemical characteristics;
In order to evaluate the biochemical characteristics of 14 substrains of Mycobacterium bovis bacillus Calmette Guérin (BCG) – Russia, Moreau, Japan, Sweden, Birkhaug, Danish, Glaxo, Mexico, Tice, Connaught, Montreal, Phipps, Australia and Pasteur – we performed eight different biochemical tests, including those for nitrate reduction, catalase, niacin accumulation, urease, Tween 80 hydrolysis, pyrazinamidase, p-amino salicylate degradation and resistance to thiophene 2-carboxylic acid hydrazide. Catalase activities of the substrains were all low. Data for nitrate reduction, niacin accumulation, Tween 80 hydrolysis, susceptibility to hydrogen peroxide and nitrate, and optimal pH for growth were all variable among these substrains. These findings suggest that the heterogeneities of biochemical characteristics are relevant to the differences in resistance of BCG substrains to environmental stress. The study also contributes to the re-evaluation of BCG substrains for use as vaccines.