Editor: Roger Buxton
The role of histone-like protein, Hlp, in Mycobacterium smegmatis dormancy
Article first published online: 14 APR 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 308, Issue 2, pages 101–107, July 2010
How to Cite
Anuchin, A. M., Goncharenko, A. V., Demina, G. R., Mulyukin, A. L., Ostrovsky, D. N. and Kaprelyants, A. S. (2010), The role of histone-like protein, Hlp, in Mycobacterium smegmatis dormancy. FEMS Microbiology Letters, 308: 101–107. doi: 10.1111/j.1574-6968.2010.01988.x
- Issue published online: 11 JUN 2010
- Article first published online: 14 APR 2010
- Received 8 February 2010; revised 30 March 2010; accepted 7 April 2010.Final version published online 21 May 2010.
- histone-like protein;
The role of histone-like protein (Hlp) in the development of a dormant state in long-incubated stationary-phase Mycobacterium smegmatis cells was studied in two models: (1) adoption of ‘nonculturable’ (NC) state, which is reversible due to resuscitation with proteinaceous resuscitation-promoting factor (Rpf) and (2) the formation of morphologically distinct, ovoid resting forms. In the first model, inactivation of the hlp gene resulted in prolongation of culturability of starved cells followed by irreversible nonculturability when mycobacterial cells were unresponsive to resuscitation with Rpf. In the second model, M. smegmatis strain with the inactivated hlp gene was able to form dormant ovoid cells, but they were less resistant to heating and UV radiation than those of wild-type strain. The susceptibility of ovoid cells produced by Δhlp mutant to these damaging factors was probably due to a less condensed state of DNA, as revealed by fluorescent microscopy and DAPI staining. Evidently, Hlp is essential for cell viability at a later stage of NC dormancy or provides a greater stability of specialized dormant forms.