Genomic and phenotypic characterization of a nonpigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530-kb megaplasmid pPag3

  1. Theo H.M. Smits1,
  2. Fabio Rezzonico1,
  3. Cosima Pelludat1,
  4. Alexander Goesmann2,
  5. Jürg E. Frey1,
  6. Brion Duffy1

Article first published online: 20 APR 2010

DOI: 10.1111/j.1574-6968.2010.01994.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 308, Issue 1, pages 48–54, July 2010

Additional Information

How to Cite

Smits, T. H., Rezzonico, F., Pelludat, C., Goesmann, A., Frey, J. E. and Duffy, B. (2010), Genomic and phenotypic characterization of a nonpigmented variant of Pantoea vagans biocontrol strain C9-1 lacking the 530-kb megaplasmid pPag3. FEMS Microbiology Letters, 308: 48–54. doi: 10.1111/j.1574-6968.2010.01994.x

Author Information

  1. 1

    Division of Plant Protection, Agroscope Changins-Wädenswil ACW, Wädenswil, Switzerland

  2. 2

    CeBiTec, University of Bielefeld, Bielefeld, Germany

*Correspondence: Theo H.M. Smits, Division of Plant Protection, Agroscope Changins-Wädenswil ACW, Schloss, Postfach, CH-8820 Wädenswil, Switzerland. Tel.: +41 44 783 6111; fax: +41 44 783 6305; e-mail: theo.smits@acw.admin.ch

Publication History

  1. Issue published online: 1 JUN 2010
  2. Article first published online: 20 APR 2010
  3. Received 29 January 2010; revised 7 April 2010; accepted 7 April 2010.Final version published online 10 May 2010.

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Keywords:

  • Pantoea vagans;
  • biological control;
  • carotenoids;
  • ampicillin;
  • autoinduction;
  • desferrioxamine

Abstract

A 530-kb megaplasmid pPag3 contributing 10.8% of the total genome of Pantoea vagans biocontrol strain C9-1 was sequenced. A rare nonpigmented variant C9-1W was obtained and shown to have lost pPag3, but retained all other plasmids (pPag1, pPag2). Phenotypic characterization of the variant confirmed the function of several annotated genes that may influence ecological fitness and efficacy. Metabolic profiling revealed important plasmid-based carbon utilization phenotypes. Plasmid loss resulted in thiamine auxotrophy, absence of carotenoid pigmentation, desferrioxamine diffusible siderophore biosynthesis, inherent ampicillin resistance and expression of AI-1 quorum-sensing signaling. This confirmed the functional expression of the corresponding genes located on pPag3 in P. vagans.

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