Editor: Paolina Garbeva
Endophytic bacterial diversity in roots of Phragmites australis in constructed Beijing Cuihu Wetland (China)
Article first published online: 17 MAY 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 309, Issue 1, pages 84–93, August 2010
How to Cite
Li, Y. H., Zhu, J. N., Zhai, Z. H. and Zhang, Q. (2010), Endophytic bacterial diversity in roots of Phragmites australis in constructed Beijing Cuihu Wetland (China). FEMS Microbiology Letters, 309: 84–93. doi: 10.1111/j.1574-6968.2010.02015.x
- Issue published online: 2 JUL 2010
- Article first published online: 17 MAY 2010
- Received 16 March 2010; revised 16 April 2010; accepted 10 May 2010.Final version published online 7 June 2010.
- endophytic bacteria;
- reed roots;
- bacterial diversity;
- 16S rRNA library;
The community structure and diversity of endophytic bacteria in reed (Phragmites australis) roots growing in the Beijing Cuihu Wetland, China was investigated using the 16S rRNA library technique. Primers 799f and 1492r were used to amplify the specific bacterial 16S rRNA fragments successfully and construct the clone library. In total, 166 individual sequences were verified by colony PCR and used to assess the diversity of endophytic bacteria in reed roots. Phylogenetic analysis revealed that 78.9% of the clones were affiliated with Proteobacteria and included all five classes. Other clones belonged to Firmicutes (9.0%), Cytophaga/Flexibacter/Bacteroids (6.6%), Fusobacteria (2.4%), and nearly 3.0% were unidentified bacteria. In Proteobacteria, the Alpha and Gamma subgroups were the most abundant, accounting for approximately 34.4% and 31.3% of all Proteobacteria, respectively, and the dominant genera included Pleomorphomonas, Azospirillum, and Aeromonas. In addition, nearly 13.6% of the Proteobacteria were very similar to some genera of sulfate-reducing bacteria (SRB) such as Dechloromonas, Desulfovibrio, and Sulfurospirillum. The bacteria in these genera are considered to play important roles in the metabolism of nitrogen, phosphorus, sulfur, and some organic compounds in wetland systems. Hence, this study demonstrates that within the diverse bacterial communities found in reed roots, endophytic strains might have a strong potential to enhance phytoremediation by reed wetlands.