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The putative RxLR effector protein SpHtp1 from the fish pathogenic oomycete Saprolegnia parasitica is translocated into fish cells

  1. Pieter Van West1,
  2. Irene De Bruijn1,2,
  3. Kirsty L. Minor1,2,
  4. Andrew J. Phillips1,2,
  5. Emma J. Robertson1,
  6. Stephan Wawra1,
  7. Judith Bain1,
  8. Victoria L. Anderson1,2,
  9. Chris J. Secombes2

Article first published online: 9 JUL 2010

DOI: 10.1111/j.1574-6968.2010.02055.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 310, Issue 2, pages 127–137, September 2010

Additional Information

How to Cite

Van West, P., De Bruijn, I., Minor, K. L., Phillips, A. J., Robertson, E. J., Wawra, S., Bain, J., Anderson, V. L. and Secombes, C. J. (2010), The putative RxLR effector protein SpHtp1 from the fish pathogenic oomycete Saprolegnia parasitica is translocated into fish cells. FEMS Microbiology Letters, 310: 127–137. doi: 10.1111/j.1574-6968.2010.02055.x

Author Information

  1. 1

    Aberdeen Oomycete Laboratory, College of Life Sciences and Medicine, Institute of Medical Sciences, University of Aberdeen – School of Medical Sciences, Foresterhill, Aberdeen, UK

  2. 2

    Scottish Fish Immunology Research Centre, Institute of Biological and Environmental Sciences, University of Aberdeen, Aberdeen, Scotland, UK

*Correspondence: Pieter van West, Aberdeen Oomycete Laboratory, College of Life Sciences and Medicine, Institute of Medical Sciences, University of Aberdeen – School of Medical Sciences, Foresterhill, Aberdeen AB25 2ZD, UK. Tel.: +01224 555 848; fax: +44 1224 555844; e-mail: p.vanwest@abdn.ac.uk

  1. Editor: Craig Shoemaker

  2. Present address: Emma J. Robertson, Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.

Publication History

  1. Issue published online: 23 AUG 2010
  2. Article first published online: 9 JUL 2010
  3. Received 18 May 2010; revised 25 June 2010; accepted 30 June 2010.Final version published online 26 July 2010.

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Keywords:

  • watermould;
  • infection;
  • protein translocation

Abstract

The fish pathogenic oomycete Saprolegnia parasitica causes the disease Saprolegniosis in salmonids and other freshwater fish, resulting in considerable economic losses in aquaculture. Very little is known about the molecular and cellular mechanisms underlying the infection process of fish pathogenic oomycetes. In order to investigate the interaction in detail, an in vitro infection assay using an Oncorhynchus mykiss (rainbow trout) cell line (RTG-2) was developed. In a zoospore/cyst cDNA library, we identified the ORF SpHtp1, which encodes a secreted protein containing an RxLR motif. Detailed expression analysis indicated that SpHtp1 is highly expressed in zoospores/cysts from S. parasitica and in the very early stages of infection on RTG-2 cells, when compared with in vitro-grown mycelium. Moreover, the protein, SpHtp1, was found to translocate into the RTG-2 trout cells, during the interaction with S. parasitica, and also when the RTG-2 cells were treated with recombinant SpHtp1 fused to a C-terminal His-tag. These findings suggest that protein translocation could play an important role in Saprolegniosis.

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