Editor: Craig Shoemaker
The putative RxLR effector protein SpHtp1 from the fish pathogenic oomycete Saprolegnia parasitica is translocated into fish cells
Article first published online: 9 JUL 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 310, Issue 2, pages 127–137, September 2010
How to Cite
Van West, P., De Bruijn, I., Minor, K. L., Phillips, A. J., Robertson, E. J., Wawra, S., Bain, J., Anderson, V. L. and Secombes, C. J. (2010), The putative RxLR effector protein SpHtp1 from the fish pathogenic oomycete Saprolegnia parasitica is translocated into fish cells. FEMS Microbiology Letters, 310: 127–137. doi: 10.1111/j.1574-6968.2010.02055.x
Present address: Emma J. Robertson, Department of Microbiology and Immunology, Albert Einstein College of Medicine, Bronx, NY 10461, USA.
- Issue published online: 23 AUG 2010
- Article first published online: 9 JUL 2010
- Received 18 May 2010; revised 25 June 2010; accepted 30 June 2010.Final version published online 26 July 2010.
Fig. S1. Alignment of the full sequences of a subset of oomycete RxLR effector proteins.
Fig. S2. Alignment of the upstream region of SpHtp1 with the conserved motif in the oomycete core promoter sequence and genome sequence of SpHtp1.
Fig. S3. Alignment of SpHtp1 with elicitin-like precursor proteins obtained by blastp analysis of SpHtp1 against nonredundant protein database in NCBI.
Fig. S4. Primer sequences used for quantitative real time RT-PCR and reference gene analysis.
Fig. S5. SpHtp1 is detected during infection.
Fig. S6. Biochemical characterization of SpHtp124-198(His)6.
Fig. S7. Uptake of SpHtp1 in fibroblast cells of the rainbow trout cell-line RTG-2.
Please note: Wiley-Blackwell is not responsible for the content or functionality of any supporting materials supplied by the authors. Any queries (other than missing material) should be directed to the corresponding author for the article.
|FML_2055_sm_supl_figs_1-7.ppt||3078K||Supporting info item|
Please note: Wiley Blackwell is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.