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Fig. S1. Alignment of the full sequences of a subset of oomycete RxLR effector proteins.

Fig. S2. Alignment of the upstream region of SpHtp1 with the conserved motif in the oomycete core promoter sequence and genome sequence of SpHtp1.

Fig. S3. Alignment of SpHtp1 with elicitin-like precursor proteins obtained by blastp analysis of SpHtp1 against nonredundant protein database in NCBI.

Fig. S4. Primer sequences used for quantitative real time RT-PCR and reference gene analysis.

Fig. S5. SpHtp1 is detected during infection.

Fig. S6. Biochemical characterization of SpHtp124-198(His)6.

Fig. S7. Uptake of SpHtp1 in fibroblast cells of the rainbow trout cell-line RTG-2.

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