Editor: Matthias Brock
Fusarium solani is responsible for mass mortalities in nests of loggerhead sea turtle, Caretta caretta, in Boavista, Cape Verde
Article first published online: 28 SEP 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 312, Issue 2, pages 192–200, November 2010
How to Cite
Sarmiento-Ramírez, J. M., Abella, E., Martín, M. P., Tellería, M. T., López-Jurado, L. F., Marco, A. and Diéguez-Uribeondo, J. (2010), Fusarium solani is responsible for mass mortalities in nests of loggerhead sea turtle, Caretta caretta, in Boavista, Cape Verde. FEMS Microbiology Letters, 312: 192–200. doi: 10.1111/j.1574-6968.2010.02116.x
- Issue published online: 19 OCT 2010
- Article first published online: 28 SEP 2010
- Accepted manuscript online: 14 SEP 2010 01:19PM EST
- Received 27 July 2010; revised 7 September 2010; accepted 8 September 2010.Final version published online 28 September 2010.
- pathogenic fungus;
- loggerhead sea turtle;
- ITS rRNA gene
The fungus Fusarium solani (Mart.) Saccardo (1881) was found to be the cause of infections in the eggs of the sea turtle species Caretta caretta in Boavista Island, Cape Verde. Egg shells with early and severe symptoms of infection, as well as diseased embryos were sampled from infected nests. Twenty-five isolates with similar morphological characteristics were obtained. Their ITS rRNA gene sequences were similar to the GenBank sequences corresponding to F. solani and their maximum identity ranged from 95% to 100%. Phylogenetic parsimony and Bayesian analyses of these isolates showed that they belong to a single F. solani clade and that they are distributed in two subclades named A and C (the latter containing 23 out of 25). A representative isolate of subclade C was used in challenge inoculation experiments to test Koch postulates. Mortality rates were c. 83.3% in challenged eggs and 8.3% in the control. Inoculated challenged eggs exhibited the same symptoms as infected eggs found in the field. Thus, this work demonstrates that a group of strains of F. solani are responsible for the symptoms observed on turtle-nesting beaches, and that they represent a risk for the survival of this endangered species.