Present address: Marie E.A. Gauthier, Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Zurich, Switzerland.
Diversity of Mycobacterium species from marine sponges and their sensitivity to antagonism by sponge-derived rifamycin-synthesizing actinobacterium in the genus Salinispora
Article first published online: 30 SEP 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 313, Issue 1, pages 33–40, December 2010
How to Cite
Izumi, H., Gauthier, M. E.A., Degnan, B. M., Ng, Y. K., Hewavitharana, A. K., Shaw, P. N. and Fuerst, J. A. (2010), Diversity of Mycobacterium species from marine sponges and their sensitivity to antagonism by sponge-derived rifamycin-synthesizing actinobacterium in the genus Salinispora. FEMS Microbiology Letters, 313: 33–40. doi: 10.1111/j.1574-6968.2010.02118.x
Editor: Jan-Ulrich Kreft
- Issue published online: 9 NOV 2010
- Article first published online: 30 SEP 2010
- Accepted manuscript online: 15 SEP 2010 12:00AM EST
- Received 10 August 2010; revised 6 September 2010; accepted 7 September 2010.Final version published online 30 September 2010.
- marine sponges;
- marine mycobacteria;
- Mycobacterium poriferae;
- Mycobacterium tuberculosis;
- Salinispora arenicola;
Eleven isolates of Mycobacterium species as well as an antimycobacterial Salinispora arenicola strain were cultured from the sponge Amphimedon queenslandica. The 16S rRNA, rpoB, and hsp65 genes from these Mycobacterium isolates were sequenced, and phylogenetic analysis of a concatenated alignment showed the formation of a large clade with Mycobacterium poriferae isolated previously from another sponge species. The separation of these Mycobacterium isolates into three species-level groups was evident from sequence similarity and phylogenetic analyses. In addition, an isolate that is phylogenetically related to Mycobacterium tuberculosis was recovered from the sponge Fascaplysinopsis sp. Several different mycobacteria thus appear to co-occur in the same sponge. An actinobacterium closely related to S. arenicola, a known producer of the antimycobacterial rifamycins, was coisolated from the same A. queenslandica specimen from which mycobacteria had been isolated. This Salinispora isolate was confirmed to synthesize rifamycin and displayed inhibitory effects against representatives from two of three Mycobacterium phylotype groups. Evidence for antagonism of sponge-derived Salinispora against sponge-derived Mycobacterium strains from the same sponge specimen and the production of antimycobacterial antibiotics by this Salinispora strain suggest that the synthesis of such antibiotics may have functions in competition between sponge microbial community members.