Editor: Peter Lund
Comparative proteomic and genetic analyses reveal unidentified mutations in Escherichia coli XL1-Blue and DH5α
Article first published online: 26 NOV 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 314, Issue 2, pages 119–124, January 2011
How to Cite
Xia, X.-X., Qian, Z.-G. and Lee, S. Y. (2011), Comparative proteomic and genetic analyses reveal unidentified mutations in Escherichia coli XL1-Blue and DH5α. FEMS Microbiology Letters, 314: 119–124. doi: 10.1111/j.1574-6968.2010.02157.x
- Issue published online: 16 DEC 2010
- Article first published online: 26 NOV 2010
- Accepted manuscript online: 9 NOV 2010 09:05AM EST
- Received 7 August 2010; revised 18 October 2010; accepted 20 October 2010.Final version published online 26 November 2010.
- Escherichia coli;
- insertion mutation
Escherichia coli has been used widely in laboratory and the biotech industry. However, the genetic and metabolic characteristics remain inadequately studied, particularly for those strains with extensive genetic manipulations that might have resulted in unknown mutations. Here, we demonstrate a comparative proteomics and genetics approach to identify unknown mutations in E. coli K-12 derivatives. The comparative proteomic and genetic analyses revealed an IS5 disruption of the kdgR gene in two commonly used derivative strains of E. coli K-12, XL1-Blue and DH5α, compared with K-12 wild-type strain W3110. In addition, a controversial deoR mutation was clarified as a wild type in E. coli DH5α using the same approach. This approach should be useful in characterizing the unknown mutations in various mutant strains developed. At the same time, comparative proteomic analysis also revealed the distinct metabolic characteristic of the two derivatives: higher biosynthetic flux to purine nucleotides. This is potentially beneficial for the synthesis of plasmid DNA.