Editor: Ezio Ricca
Evaluation of the ability of Bifidobacterium longum to metabolize human intestinal mucus
Article first published online: 24 NOV 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 314, Issue 2, pages 125–130, January 2011
How to Cite
Ruiz, L., Gueimonde, M., Couté, Y., Salminen, S., Sanchez, J.-C., de los Reyes-Gavilán, C. G. and Margolles, A. (2011), Evaluation of the ability of Bifidobacterium longum to metabolize human intestinal mucus. FEMS Microbiology Letters, 314: 125–130. doi: 10.1111/j.1574-6968.2010.02159.x
- Issue published online: 16 DEC 2010
- Article first published online: 24 NOV 2010
- Accepted manuscript online: 9 NOV 2010 09:07AM EST
- Received 1 October 2010; accepted 22 October 2010.Final version published online 24 November 2010.
- Bifidobacterium longum;
- human intestinal mucus;
The ability of Bifidobacterium longum to use intestinal mucus as a metabolizable source was characterized. Bifidobacterium longum biotype longum NCIMB8809 was grown in a chemically semi-defined medium supplemented with human intestinal mucus, and the cytoplasmic protein profiles and several glycosyl hydrolase activities were analysed and compared with those obtained from the same bacterium grown in the absence of mucus. We were able to identify 22 different proteins in the cytoplasmic fraction, of which nine displayed a different concentration in the presence of mucus. Among the proteins whose concentrations varied, we found specific enzymes that are involved in the response to different environmental conditions, and also proteins that mediate interaction with mucus in bacteria. Significant changes in some glycoside-hydrolysing activities were also detected. In addition, stable isotope labelling of amino acids in cell culture demonstrated that B. longum incorporates leucine from the glycoprotein matrix of mucin within its proteins. This study provides the first proteomic data regarding the interaction of B. longum with intestinal mucus, and contributes to the understanding of the behaviour of this intestinal species in its natural ecological niche.