Editor: Akio Nakane
HtpS, a novel immunogenic cell surface-exposed protein of Streptococcus suis, confers protection in mice
Article first published online: 6 DEC 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 314, Issue 2, pages 174–182, January 2011
How to Cite
Shao, Z., Pan, X., Li, X., Liu, W., Han, M., Wang, C., Wang, J., Zheng, F., Cao, M. and Tang, J. (2011), HtpS, a novel immunogenic cell surface-exposed protein of Streptococcus suis, confers protection in mice. FEMS Microbiology Letters, 314: 174–182. doi: 10.1111/j.1574-6968.2010.02162.x
- Issue published online: 16 DEC 2010
- Article first published online: 6 DEC 2010
- Accepted manuscript online: 17 NOV 2010 06:37AM EST
- Received 21 September 2010; revised 8 November 2010; accepted 9 November 2010.Final version published online 6 December 2010.
- Streptococcus suis serotype 2 (S. suis 2);
- vaccine candidate
Streptococcal histidine triad protein was identified recently as a cell surface-associated protein family. Five members of this family (PhtA, PhtB, PhtD, PhtE and HtpA), derived from Streptococcus pneumoniae and Streptococcus pyogenes, have been shown as antigens that confer protection to the host on infection. In this report, a gene sequence highly homologous to htpA and phtD (designated htpS, the histidine triad protein of Streptococcus suis) was identified from S. suis 2 Chinese strain 05ZYH33. Our data revealed that htpS is extremely conserved in S. suis 2 and widely distributed in 83% (29/35) of 35 S. suis serotypes. It was also demonstrated by Western blot and flow cytometry that HtpS is a cell surface-associated protein that was expressed during S. suis 2 infection. An antibody against HtpS could increase the deposition of human complement 3 on S. suis 2 and also enhance the clearance of S. suis 2 in whole blood. In addition, mice could be immunized against S. suis 2 infection and were well protected after immunization with recombinant HtpS.