Editor: Stephen Smith
Detergents enhance EspB secretion from Escherichia coli strains harboring the locus for the enterocyte effacement (LEE) gene
Article first published online: 22 DEC 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 315, Issue 2, pages 109–114, February 2011
How to Cite
Nakasone, N., Toma, C., Higa, N., Koizumi, Y., Ogura, Y. and Suzuki, T. (2011), Detergents enhance EspB secretion from Escherichia coli strains harboring the locus for the enterocyte effacement (LEE) gene. FEMS Microbiology Letters, 315: 109–114. doi: 10.1111/j.1574-6968.2010.02176.x
- Issue published online: 14 JAN 2011
- Article first published online: 22 DEC 2010
- Accepted manuscript online: 1 DEC 2010 06:53AM EST
- Received 26 October 2010; revised 23 November 2010; accepted 23 November 2010.Final version published online 22 December 2010.
- EspB secretion;
- detergent enhancement;
- EPEC and STEC
The effects of detergents (cholic acid, deoxycholic acid, Triton X-100, and Nonidet P-40) on the secretion of EspB from the locus for enterocyte effacement (LEE) gene-positive Escherichia coli strains were examined. Clinical isolates of eight EPEC strains and seven STEC strains were used to detect EspB after they had been cultivated in Luria–Bertani (LB) broth containing one of the detergents. When the bacteria were cultured in LB broth supplemented with one of the detergents, the amount of EspB produced was increased by 2–32-fold depending on the detergent and the strain used. EspB was detected in all strains when they were cultured in LB broth containing all of the detergents. The results obtained in this study can be applied to immunological diagnostic methods for detecting EspB and also to the production of EspB for research purposes.