Editor: Christiane Dahl
Flux-based analysis of sulfur metabolism in desulfurizing strains of Rhodococcus erythropolis
Article first published online: 23 DEC 2010
© 2010 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 315, Issue 2, pages 115–121, February 2011
How to Cite
Aggarwal, S., Karimi, I. A. and Lee, D. Y. (2011), Flux-based analysis of sulfur metabolism in desulfurizing strains of Rhodococcus erythropolis. FEMS Microbiology Letters, 315: 115–121. doi: 10.1111/j.1574-6968.2010.02179.x
- Issue published online: 14 JAN 2011
- Article first published online: 23 DEC 2010
- Accepted manuscript online: 3 DEC 2010 09:55AM EST
- Received 26 August 2010; revised 21 October 2010; accepted 29 November 2010.Final version published online 23 December 2010.
- stoichiometric model;
- sulfur metabolism;
- Rhodococcus erythropolis
Rhodococcus erythropolis has been studied widely for potential applications in biodesulfurization. Previous works have been largely experimental with an emphasis on the characterization and genetic engineering of desulfurizing strains for improved biocatalysis. A systems modeling approach that can complement these experimental efforts by providing useful insights into the complex interactions of desulfurization reactions with various other metabolic activities is absent in the literature. In this work, we report the first attempt at reconstructing a flux-based model to analyze sulfur utilization by R. erythropolis. The model includes the 4S pathway for dibenzothiophene (DBT) desulfurization. It predicts closely the growth rates reported by two independent experimental studies, and gives a clear and comprehensive picture of the pathways that assimilate the sulfur from DBT into biomass. In addition, it successfully elucidates that sulfate promotes higher cell growth than DBT and its presence in the medium reduces DBT desulfurization rates. A study using eight carbon sources suggests that ethanol and lactate yield higher cell growth and desulfurization rates than citrate, fructose, glucose, gluconate, glutamate, and glycerol.