Editor: Reggie Lo
Functional analysis of Borrelia burgdorferi uvrA in DNA damage protection
Article first published online: 17 FEB 2011
© 2011 New York Medical College. FEMS Microbiology Letters © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd
FEMS Microbiology Letters
Volume 317, Issue 2, pages 172–180, April 2011
How to Cite
Sambir, M., Ivanova, L. B., Bryksin, A. V., Godfrey, H. P. and Cabello, F. C. (2011), Functional analysis of Borrelia burgdorferi uvrA in DNA damage protection. FEMS Microbiology Letters, 317: 172–180. doi: 10.1111/j.1574-6968.2011.02226.x
Present address: Anton V. Bryksin, Department of Biochemistry, Rollins Research Center, Emory University School of Medicine, 1510 Clifton Road, Atlanta, GA 30322, USA.
- Issue published online: 22 MAR 2011
- Article first published online: 17 FEB 2011
- Accepted manuscript online: 27 JAN 2011 02:26PM EST
- Received 3 October 2010; revised 29 December 2010; accepted 18 January 2011., Final version published online 17 February 2011.
Vol. 339, Issue 1, 75, Article first published online: 21 DEC 2012
- Borrelia burgdorferi;
- Lyme disease;
- DNA damage;
- nucleotide excision repair;
- UV radiation
Bacterial pathogens face constant challenges from DNA-damaging agents generated by host phagocytes. Although Borrelia burgdorferi appears to have much fewer DNA repair enzymes than pathogens with larger genomes, it does contain homologues of uvrA and uvrB (subunits A and B of excinuclease ABC). As a first step to exploring the physiologic function of uvrABbu and its possible role in survival in the host in the face of DNA-damaging agents, a partially deleted uvrA mutant was isolated by targeted inactivation. While growth of this mutant was markedly inhibited by UV irradiation, mitomycin C (MMC) and hydrogen peroxide at doses that lacked effect on wild-type B. burgdorferi, its response to pH 6.0–6.8 and reactive nitrogen intermediates was similar to that of the wild-type parental strain. The sensitivity of the inactivation mutant to UV irradiation, MMC and peroxide was complemented by an extrachromosomal copy of uvrABbu. We conclude that uvrABbu is functional in B. burgdorferi.