Editor: Peter Lund
Functional genomics of the initial phase of cold adaptation of Pseudomonas putida KT2440
Article first published online: 1 MAR 2011
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 318, Issue 1, pages 47–54, May 2011
How to Cite
Frank, S., Schmidt, F., Klockgether, J., Davenport, C. F., Gesell Salazar, M., Völker, U. and Tümmler, B. (2011), Functional genomics of the initial phase of cold adaptation of Pseudomonas putida KT2440. FEMS Microbiology Letters, 318: 47–54. doi: 10.1111/j.1574-6968.2011.02237.x
- Issue published online: 4 APR 2011
- Article first published online: 1 MAR 2011
- Accepted manuscript online: 9 FEB 2011 08:26AM EST
- Received 5 October 2010; revised 26 January 2011; accepted 1 February 2011., Final version published online 1 March 2011.
- Pseudomonas putida;
- cold adaptation;
The cold stress response of Pseudomonas putida KT2440 was investigated by genomewide deep cDNA sequencing and gel-free MS-based protein profiling. Transcriptome and proteome profiles were assessed at 30 °C and 2 h after a downshift from 30 to 10 °C. Pseudomonas putida adapted to lower ambient temperature by the activation of ribosome-associated functional modules that facilitate translational efficiency. The outer membrane profile was reorganized, anabolic pathways and core as well as energy metabolism were repressed and the alginate regulon and sugar catabolism were activated. At the investigated early time point of cold adaptation, the transcriptome was reprogrammed in almost all functional categories, but the protein profile had still not adapted to the change of living conditions in the cold.