Editor: Ezio Ricca
A flagellin-producing Lactococcus strain: interactions with mucin and enteropathogens
Article first published online: 8 MAR 2011
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 318, Issue 2, pages 101–107, May 2011
How to Cite
Sánchez, B., López, P., González-Rodríguez, I., Suárez, A., Margolles, A. and Urdaci, M. C. (2011), A flagellin-producing Lactococcus strain: interactions with mucin and enteropathogens. FEMS Microbiology Letters, 318: 101–107. doi: 10.1111/j.1574-6968.2011.02244.x
- Issue published online: 7 APR 2011
- Article first published online: 8 MAR 2011
- Accepted manuscript online: 16 FEB 2011 07:27AM EST
- Received 2 February 2011; revised 11 February 2011; accepted 11 February 2011., Final version published online 8 March 2011.
Bacillus cereus CH is a probiotic strain used in human nutrition whose adhesion to mucin is dependent on its surface-associated flagellin. Flagellins from the surface of several probiotic Bacillus strains were efficiently extracted with 5 M LiCl and identified by peptide fingerprinting. Based on the proteomic analysis, cloning of the gene coding for the flagellin of B. cereus CH was performed in the lactococcal vector pNZ8110 under the control of a nisin-inducible promoter. The resulting strain, Lactococcus lactis CH, produced a surface-associated flagellin after 6 h of induction with nisin. The recombinant Lactococcus strain adhered strongly to mucin-coated polystyrene plates, whilst inhibiting competitively the adhesion of the pathogens Escherichia coli LMG2092 and Salmonella enterica ssp. enterica LMG15860 to the same molecule. Strain CH could be used in further experimentation for the characterization of the molecular mechanism of action of this probiotic B. cereus CH flagellin.