Editor: David Clarke
Acanthamoeba sp. promotes the survival and growth of Acinetobacter baumanii
Article first published online: 31 MAR 2011
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 319, Issue 1, pages 19–25, June 2011
How to Cite
Cateau, E., Verdon, J., Fernandez, B., Hechard, Y. and Rodier, M.-H. (2011), Acanthamoeba sp. promotes the survival and growth of Acinetobacter baumanii. FEMS Microbiology Letters, 319: 19–25. doi: 10.1111/j.1574-6968.2011.02261.x
- Issue published online: 21 APR 2011
- Article first published online: 31 MAR 2011
- Accepted manuscript online: 11 MAR 2011 01:58PM EST
- Received 18 February 2011; revised 4 March 2011; accepted 4 March 2011., Final version published online 31 March 2011.
- Acinetobacter baumanii;
Acinetobacter baumanii, which may be found in water, is an important emerging hospital-acquired pathogen. Free-living amoebae can be recovered from the same water networks, and it has been shown that these protozoa may support the growth of other bacteria. In this paper, we have studied potential relationships between A. baumanii and Acanthamoeba species. Two strains of A. baumanii isolated from hospital water were co-cultivated with the trophozoites or supernatants of two free-living amoebae strains: Acanthamoeba castellanii or Acanthamoeba culbertsoni. Firstly, the presence of the amoebae or their supernatants induced a major increase in A. baumanii growth, compared with controls. Secondly, A. baumanii affected only the viability of A. culbertsonii, with no effect on A. castellanii. Electron microscopy observations of the cultures investigating the bacterial location in the protozoa showed persistence of the bacteria within cyst wall even after 60 days of incubation. In our study, the survival and growth of A. baumanii could be favored by Acanthamoeba strains. Special attention should consequently be paid to the presence of free-living amoebae in hospital water systems, which can promote A. baumanii persistence.