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Fig. S1. Viable cell count of Ant5-2 after a single freeze–thaw cycle.

Fig. S2. Autoradiogram of 35S-methionine-labeled total cellular proteins from Ant5-2 cultures at different temperatures.

Fig. S3. Multiple sequence alignment of deduced amino acid sequence of the cold shock protein CspD from Ant5-2 with the cold shock transcriptional regulator sequence from J. lividum, CspE from H. arsenicoxydans, CspD1 from Janthinobacterium sp. Marseille, cold-shock DNA-binding protein family protein from T. denitrificans ATCC 25259, cold-shock DNA-binding protein family protein from D. aromatica RCB, CspD from B. phymatum STM815, CspA from N. meningitidis Z2491, cold shock protein from R. pickettii 12D and CspE from C. violaceum ATCC 12472.

Fig. S4. The agarose gel (1% w/v) showing the results of PCR amplification with CSPU5 and CSPU3 universal primers CSPU5 and CSPU3.

Fig. S5. SDS-polyacrylamide gel (12%, w/v) electrophoresis showing purified CspD of Ant5-2 expressed in Escherichia coli.

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