Editor: Roger Buxton
The wag31 gene of Mycobacterium tuberculosis is positively regulated by the stringent response
Article first published online: 6 MAY 2011
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 319, Issue 2, pages 153–159, June 2011
How to Cite
Dahl, J. L. and Lau Bonilla, D. (2011), The wag31 gene of Mycobacterium tuberculosis is positively regulated by the stringent response. FEMS Microbiology Letters, 319: 153–159. doi: 10.1111/j.1574-6968.2011.02278.x
- Issue published online: 17 MAY 2011
- Article first published online: 6 MAY 2011
- Accepted manuscript online: 6 APR 2011 08:20AM EST
- Received 10 February 2011; revised 21 March 2011; accepted 30 March 2011., Final version published online 6 May 2011.
- Mycobacterium tuberculosis;
- Mycobacterium smegmatis;
- stringent response;
The stringent response of Mycobacterium tuberculosis is coordinated by Rel and is required for full virulence in animal models. A serological-based approach identified Wag31Mtb as a protein that is upregulated in M. tuberculosis in a rel-dependent manner. This positive regulation was confirmed by analysis of M. tuberculosis mRNA expression. Mycobacterium smegmatis was used to confirm that the expression of wag31Mtb from its native promoter is positively regulated by the stringent response. Furthermore, elevated wag31Mtb expression in M. smegmatis drastically alters the cell-surface hydrophobic properties.