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Kinetic characterization and gene expression of adenosine deaminase in intact trophozoites of Trichomonas vaginalis

  1. Marina Weizenmann1,
  2. Amanda Piccoli Frasson1,
  3. Muriel Primon de Barros1,
  4. Patrícia de Brum Vieira1,
  5. Denis Broock Rosemberg2,
  6. Geraldo Attilio De Carli3,
  7. Maurício Reis Bogo4,
  8. Carla Denise Bonan5,
  9. Tiana Tasca1

Article first published online: 26 APR 2011

DOI: 10.1111/j.1574-6968.2011.02283.x

Issue

FEMS Microbiology Letters

FEMS Microbiology Letters

Volume 319, Issue 2, pages 115–124, June 2011

Additional Information

How to Cite

Weizenmann, M., Frasson, A. P., de Barros, M. P., Vieira, P. d. B., Rosemberg, D. B., De Carli, G. A., Bogo, M. R., Bonan, C. D. and Tasca, T. (2011), Kinetic characterization and gene expression of adenosine deaminase in intact trophozoites of Trichomonas vaginalis. FEMS Microbiology Letters, 319: 115–124. doi: 10.1111/j.1574-6968.2011.02283.x

Author Information

  1. 1

    Laboratório de Pesquisa em Parasitologia, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

  2. 2

    Departamento de Bioquímica, ICBS, Universidade Federal do Rio Grande do Sul, Porto Alegre, RS, Brazil

  3. 3

    Instituto de Geriatria e Gerontologia, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil

  4. 4

    Laboratório de Biologia Genômica e Molecular, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil

  5. 5

    Laboratório de Neuroquímica e Psicofarmacologia, Faculdade de Biociências, Pontifícia Universidade Católica do Rio Grande do Sul, Porto Alegre, RS, Brazil

*Correspondence: Tiana Tasca, Laboratório de Pesquisa em Parasitologia, Faculdade de Farmácia, Universidade Federal do Rio Grande do Sul, Av. Ipiranga 2752, 90610-000, Porto Alegre, RS, Brazil. Tel.: +55 51 3308 5325; fax: +55 51 3308 5437; e-mail: tiana.tasca@ufrgs.br

  1. Editor: Albert Descoteaux

Publication History

  1. Issue published online: 17 MAY 2011
  2. Article first published online: 26 APR 2011
  3. Accepted manuscript online: 8 APR 2011 10:37AM EST
  4. Received 19 February 2011; accepted 11 March 2011., Final version published online 26 April 2011.

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Keywords:

  • Trichomonas vaginalis;
  • adenosine deaminase;
  • intact trophozoites;
  • adenosine;
  • inosine

Abstract

Trichomonas vaginalis is a parasite that resides in the human urogenital tract and causes trichomonosis, the most prevalent nonviral sexually transmitted disease. Nucleoside triphosphate diphosphohydrolase (NTPDase), which hydrolyzes extracellular di- and triphosphate nucleotides, and ecto-5′-nucleotidase, which hydrolyzes AMP, have been characterized in T. vaginalis. The aim of this study was to characterize the adenosine deaminase (ADA) activity in intact trophozoites of T. vaginalis. A strong inhibition in adenosine deamination was observed in the presence of calcium and magnesium, which was prevented by EDTA. The apparent KM value for adenosine was 1.13 ± 0.07 mM. The calculated Vmax was 2.61 ± 0.054 nmol NH3 min−1 mg−1 protein. Adenosine deamination was inhibited in the presence of erythro-9-(2-hydroxy-3-nonyl)adenine. Semi-quantitative reverse transcriptase-PCR experiments were performed and both ADA-related genes ada(125) and ada(231) mRNA were expressed, although ada(231) in higher quantity when compared with the ada(125) : α-tubulin ratio. Furthermore, a phylogenetic analysis showed that the T. vaginalis sequences formed a clade with Entamoeba histolytica and Dictyostelium discoideum sequences, and it strongly suggests homologous functions in the T. vaginalis genome. The presence of ADA activity in T. vaginalis may be important to modulate the adenosine/inosine levels during infection and, consequently, to maintain the anti-inflammatory properties through different nucleoside-signalling mechanisms.

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