Present address: A. Nicole Trombert, Centro de Genómica y Bioinformática, Instituto de Biotecnología, Universidad Mayor, Santiago, Chile.
A holin remnant protein encoded by STY1365 is involved in envelope stability of Salmonella enterica serovar Typhi
Article first published online: 6 JUN 2011
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 321, Issue 1, pages 58–66, August 2011
How to Cite
Rodas, P. I., Trombert, A. N. and Mora, G. C. (2011), A holin remnant protein encoded by STY1365 is involved in envelope stability of Salmonella enterica serovar Typhi. FEMS Microbiology Letters, 321: 58–66. doi: 10.1111/j.1574-6968.2011.02310.x
Editor: Stephen Smith
- Issue published online: 4 JUL 2011
- Article first published online: 6 JUN 2011
- Accepted manuscript online: 18 MAY 2011 10:12AM EST
- Received 19 April 2011; revised 10 May 2011; accepted 11 May 2011., Final version published online 6 June 2011.
- inner membrane
We characterized STY1365, a small ORF of Salmonella enterica serovar Typhi. This 174-bp ORF encodes a putative product of 57 amino acid residues with a premature stop codon. Nevertheless, bioinformatic analyses revealed that the predicted product of STY1365 has similarity to putative holin genes of Escherichia coli and bacteriophage ΦP27. STY1365 showed a high-level expression at the early log phase and a small corresponding protein product was detected mainly in the inner membrane fraction. Cloning of STY1365 in pSU19 mid-copy-vector produced retardation in S. Typhi growth, increased cell permeability to crystal violet and altered the inner membrane protein profile. Similar results were obtained when STY1365 was induced with isopropyl-β-d-thio-galactoside in pCC1™ single-copy vector. Our results support the fact that S. Typhi STY1365 encodes a holin remnant protein that is involved in the stability of the bacterial envelope.