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Fig. S1. Eight known metabolites that have been linked to specific PKS genes in Aspergillus nidulans.

Fig. S2. A graphical illustration of the procedure used to make the gene targeting fragments for the PKS deletions.

Fig. S3. Procedure for diagnostic PCR.

Fig. S4. Chromosome map showing the position of the 32 PKS genes.

Fig. S5. Verification that the polyketide is absent in selected deletion mutants.

Fig. S6. Positive mode extracted ion chromatograms for the mdpGΔ strain cultivated on RTO.

Fig. S7. Additional polyketides that were detected in metabolite extracts in this study.

Fig. S8. The mutants orsAΔ and AN7903Δ lack production of violaceols.

Fig. S9. Extracted ion chromatograms of metabolic extracts from the reference and ausAΔ strains.

Fig. S10.1H NMR spectrum of 3,5-dimethylorsellinic acid in dimethyl sulfoxide-d6.

Fig. S11.1H NMR spectrum of dehydroaustinol in CDCl3.

Fig. S12.1H NMR spectrum of arugosin A open form in dimethyl sulfoxide-d6.

Table S1. PCR primers for Gateway assembly.

Table S2. Oligonucleotide primers for diagnostic PCR.

Table S3. Additional oligonucleotides used in the study.

Table S4. The constructed Aspergillus nidulans strains have been deposited into the IBT Culture Collection.

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