Editor: Richard Staples
A genome-wide polyketide synthase deletion library uncovers novel genetic links to polyketides and meroterpenoids in Aspergillus nidulans
Article first published online: 27 JUN 2011
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 321, Issue 2, pages 157–166, August 2011
How to Cite
Nielsen, M. L., Nielsen, J. B., Rank, C., Klejnstrup, M. L., Holm, D. K., Brogaard, K. H., Hansen, B. G., Frisvad, J. C., Larsen, T. O. and Mortensen, U. H. (2011), A genome-wide polyketide synthase deletion library uncovers novel genetic links to polyketides and meroterpenoids in Aspergillus nidulans. FEMS Microbiology Letters, 321: 157–166. doi: 10.1111/j.1574-6968.2011.02327.x
- Issue published online: 14 JUL 2011
- Article first published online: 27 JUN 2011
- Accepted manuscript online: 9 JUN 2011 10:30AM EST
- Received 14 April 2011; revised 26 May 2011; accepted 27 May 2011., Final version published online 27 June 2011.
Fig. S1. Eight known metabolites that have been linked to specific PKS genes in Aspergillus nidulans.
Fig. S2. A graphical illustration of the procedure used to make the gene targeting fragments for the PKS deletions.
Fig. S3. Procedure for diagnostic PCR.
Fig. S4. Chromosome map showing the position of the 32 PKS genes.
Fig. S5. Verification that the polyketide is absent in selected deletion mutants.
Fig. S6. Positive mode extracted ion chromatograms for the mdpGΔ strain cultivated on RTO.
Fig. S7. Additional polyketides that were detected in metabolite extracts in this study.
Fig. S8. The mutants orsAΔ and AN7903Δ lack production of violaceols.
Fig. S9. Extracted ion chromatograms of metabolic extracts from the reference and ausAΔ strains.
Fig. S10.1H NMR spectrum of 3,5-dimethylorsellinic acid in dimethyl sulfoxide-d6.
Fig. S11.1H NMR spectrum of dehydroaustinol in CDCl3.
Fig. S12.1H NMR spectrum of arugosin A open form in dimethyl sulfoxide-d6.
Table S1. PCR primers for Gateway assembly.
Table S2. Oligonucleotide primers for diagnostic PCR.
Table S3. Additional oligonucleotides used in the study.
Table S4. The constructed Aspergillus nidulans strains have been deposited into the IBT Culture Collection.
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