Editor: Hermann Bothe
The purL gene of Bacillus subtilis is associated with nematicidal activity
Article first published online: 21 JUL 2011
© 2011 Nanjing Agricultural University. FEMS Microbiology Letters © 2011 Federation of European Microbiological Societies. Published by Blackwell Pulishing Ltd
FEMS Microbiology Letters
Volume 322, Issue 2, pages 99–107, September 2011
How to Cite
Xia, Y., Xie, S., Ma, X., Wu, H., Wang, X. and Gao, X. (2011), The purL gene of Bacillus subtilis is associated with nematicidal activity. FEMS Microbiology Letters, 322: 99–107. doi: 10.1111/j.1574-6968.2011.02336.x
- Issue published online: 11 AUG 2011
- Article first published online: 21 JUL 2011
- Accepted manuscript online: 14 JUN 2011 10:32AM EST
- Received 24 April 2011; revised 3 June 2011; accepted 6 June 2011, Final version published online 21 July 2011.
- plant-parasitic nematodes;
- nematicidal-related genes;
Parasitic nematodes of plants are important plant pathogens that represent a significant financial burden on agriculture. This study evaluated the efficacy of Bacillus spp. as nematode biocontrol agents and identified Bacillus genes associated with nematicidal activity. Culture by products of Bacillus subtilis strains OKB105 and 69 and Bacillus amyloliquefaciens strains FZB42 and B3 were used to treat Aphelenchoides besseyi, Ditylenchus destructor, Bursaphelenchus xylophilus and Meloidogyne javanica, respectively. The highest mortality rates were observed at 12 h when combinations of either A. besseyi/B3, D. destructor/OKB105, B. xylophilus/69 or M. javanica/OKB105 resulted in 10.6%, 27.6%, 35.6% and 100% mortality rates, respectively. Supernatant analysis demonstrated that the nematicidal active ingredients of strain OKB105, with a molecular weight of <1000 Da, were nonproteinaceous, heat and cold resistant, highly polar and could be evaporated but not extracted by some organic solvents. To identify nematicidal-related genes, 2000 OKB105 mutants were generated using the TnYLB-1 transposon. Mutant M1 lost nematicidal activity by 72 h and inverse PCR results demonstrated disruption of the purL gene. Nematicidal activity was restored when M1 mutant was complemented with either plasmid pMA5-purL or pUC18-purL, demonstrating a role for purL in mediating nematicidal activity.