Editor: Dr. Andreas Stolz
Active and silent members in the mlr gene cluster of a microcystin-degrading bacterium isolated from Lake Taihu, China
Article first published online: 19 JUL 2011
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 322, Issue 2, pages 108–114, September 2011
How to Cite
Jiang, Y., Shao, J., Wu, X., Xu, Y. and Li, R. (2011), Active and silent members in the mlr gene cluster of a microcystin-degrading bacterium isolated from Lake Taihu, China. FEMS Microbiology Letters, 322: 108–114. doi: 10.1111/j.1574-6968.2011.02337.x
- Issue published online: 11 AUG 2011
- Article first published online: 19 JUL 2011
- Accepted manuscript online: 15 JUN 2011 10:19AM EST
- Received 2 May 2011; revised 9 June 2011; accepted 10 June 2011, Final version published online 19 July 2011.
- degradation efficiency;
- gene expression;
- frame-shift mutation
The microcystin-degrading genes, mlr, are important participants in the degradation process of hepatotoxic microcystins for several bacterial species. However, their expression status during degrading microcystins is still unknown. In order to study this expression process, we isolated a novel microcystin-degrading bacterial strain, sequenced its mlr gene cluster and examined the expression of the mlrA gene at different concentrations of microcystin LR. The expression of mlrA increased slightly at 0.4 mg L−1, and was significantly upregulated at 2.0 mg L−1. Frameshift mutations were found in the mlrB* gene, and the mRNA of mlrB* could not be detected in the total RNA extracts of Novosphingobium sp. THN1. We conclude that mlrA is actively involved in the microcystin–degrading process, but mlrB* has lost its activity in this bacterial strain.