Identification of the srtC1 transcription start site and catalytically essential residues required for Actinomyces oris T14V SrtC1 activity
Article first published online: 27 JUL 2011
FEMS Microbiology Letters © 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. No claim to original US government works
FEMS Microbiology Letters
Volume 322, Issue 2, pages 115–122, September 2011
How to Cite
Chen, P. and Leung, K. P. (2011), Identification of the srtC1 transcription start site and catalytically essential residues required for Actinomyces oris T14V SrtC1 activity. FEMS Microbiology Letters, 322: 115–122. doi: 10.1111/j.1574-6968.2011.02338.x
- Issue published online: 11 AUG 2011
- Article first published online: 27 JUL 2011
- Accepted manuscript online: 15 JUN 2011 10:19AM EST
- Received 27 April 2011; revised 9 June 2011; accepted 10 June 2011, Final version published online 27 July 2011.
In Actinomyces oris T14V, sortase SrtC1 mediates the assembly of type 1 fimbriae. We analyzed the effects of the conserved residues (H184, H204, F213, Y236, L263, T265, C266 and R275) on the SrtC1 activity by site-directed mutagenesis. We identified three essential conserved residues (H204, Y236 and C266) that are critical for the assembly of type 1 fimbriae in this organism. rapid amplification of cDNA ends analyses and reverse transcriptase-PCR results indicate that srtC1 was transcribed together with the putative adhesin gene fimQ and major structural subunit gene fimP as a single polycistronic mRNA.