Present addresses: Györgyi Nyerges, Department of Biology, Pacific University, 2043 College Way, Forest Grove, OR 97116, USA. Amisha T. Poret-Peterson, School of Earth and Space Exploration, Arizona State University, PO Box 871404, Tempe, AZ 85287-1404, USA.
Model of the molecular basis for hydroxylamine oxidation and nitrous oxide production in methanotrophic bacteria
Article first published online: 13 JUL 2011
© 2011 Federation of European Microbiological Societies. Published by Blackwell Publishing Ltd. All rights reserved
FEMS Microbiology Letters
Volume 322, Issue 1, pages 82–89, September 2011
How to Cite
Campbell, M. A., Nyerges, G., Kozlowski, J. A., Poret-Peterson, A. T., Stein, L. Y. and Klotz, M. G. (2011), Model of the molecular basis for hydroxylamine oxidation and nitrous oxide production in methanotrophic bacteria. FEMS Microbiology Letters, 322: 82–89. doi: 10.1111/j.1574-6968.2011.02340.x
Editor: J. Murrell
- Issue published online: 22 JUL 2011
- Article first published online: 13 JUL 2011
- Accepted manuscript online: 17 JUN 2011 09:03AM EST
- Received 4 April 2011; revised 6 June 2011; accepted 11 June 2011., Final version published online 6 June 2011.
- nitrous oxide;
- hydroxylamine oxidoreductase;
- nitric oxide reductase;
- ammonia oxidation;
- Methylococcus capsulatus Bath
Many methane-oxidizing bacteria (MOB) have been shown to aerobically oxidize ammonia and hydroxylamine (NH2OH) to produce nitrite and nitrous oxide (N2O). Genome sequences of alphaproteobacterial, gammaproteobacterial, and verrucomicrobial methanotrophs revealed the presence of haoAB, cytL, cytS, nirS or nirK, and norCB genes that may be responsible for N2O production, and additional haoAB genes were sequenced from two strains of Methylomicrobium album. The haoAB genes of M. album ATCC 33003 were inducible by ammonia and NH2OH, similar to haoAB induction by ammonia in Methylococcus capsulatus Bath. Increased expression of genes encoding nitric oxide reductase (cNOR; norCB) was measured upon exposure of M. capsulatus Bath to NaNO2 and NO-releasing sodium nitroprusside. Only incubations of M. capsulatus Bath with methane, ammonia, and nitrite produced N2O. The data suggest a possible pathway of nitrite reduction to NO by reversely operating NH2OH oxidoreductase and NO reduction to N2O by cNOR independently or in conjunction with ammonia-induced enzymes (i.e. HAO or cytochrome c′-β). Results of this study show that MOB likely have diverse mechanisms for nitrogen oxide metabolism and detoxification of NH2OH that involve conventional and unconventional enzymes.